Bioremediation of Waste Water from Pharmaceutical Industry by Bacteria (Bacillus subtilis)

Abstract

This work aimed to characterize pharmaceutical waste generated in Sam Ace Pharmaceutical in Ede, Osun-state, and assess wastewater treatment plant performance by bacteria (Bacillus subtilis) and the feasibility of wastewater reuse. Freshly discharged pharmaceutical wastewater was collected and analyzed for the physicochemical parameters such as Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Dissolved Solid (TDS), Nitrate, Phosphate, Magnesium, Calcium, Zinc, and Copper by standard methods. 190ml of the sterilized FPWWS was inoculated with pharmaceutical wastewater for two weeks and determined the physicochemical parameters at a 7-day interval. The results observed for raw, bio-treated and removal efficiency showed: BOD(200 mg-1, 90 mg-1 , 45 mg-1 and 55%, 75.5%), COD(395 mg-1, 330 mg-1, 150 mg-1 and 16.46 %, 62.03%), TDS(11200 mg-1, 250 mg-1, 130 mg-1 and 79.17%, 89.17%), Nitrate (165 mg-1, 88 mg-1, 43 mg-1 and 46.67%, 73.94%) phosphate (31 mg-1, 18 mg-1, 6 mg-1, and 41.94%, 80.65%), magnesium (75 mg-1, 55 mg-1, 17 mg-1, and 22.67%, 77.33%), calcium (80 mg-1, 57 mg-1, 25 mg-1, and 28.75%, 68.75%), zinc (0.05 mg-1, 0.03 mg-1, 0.001 mg-1, and 40%, 98.6%), copper (0.06, 0.02, 0.001) Bacteria (Bacillus sp.) showed a potential removal of pollutants and other wastes from the fish pond wastewater.